8WEC

Crystal structure of Arabidopsis thaliana MIK2 ectodomain in complex with BAK1 ectodomain and SCOOP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-09-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	73.004, 178.690, 144.161
Unit cell angles	90.00, 90.79, 90.00

Refinement procedure

Resolution	46.150 - 3.100
R-factor	0.2455
Rwork	0.245
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.794
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX ((1.20.1_4487: ???))
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.170
High resolution limit [Å]	3.100	3.100
Number of reflections	66926	6711
<I/σ(I)>	7.5
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.990	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.9	289	0.1 M MES pH 5.9, 22% PEG200, 7% PEG 3000