8WEB

Crystal structure of Arabidopsis thaliana MIK2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-09-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	62.127, 107.889, 164.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.130 - 1.950
R-factor	0.1725
Rwork	0.171
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.268
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((1.20.1_4487: ???))
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	81379	6952
<I/σ(I)>	32.9
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	1.000	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.4	289	0.2 M Sodium acetate, 0.1 M Tris pH 7.4 and 18% (w/v) PEG 3350