8WDX

Crystal structure of human FABP4 complexed with C3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	35.645, 54.889, 74.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.190 - 1.650
R-factor	0.2022
Rwork	0.201
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.762
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.190	1.709
High resolution limit [Å]	1.650	1.650
Number of reflections	18385	1791
<I/σ(I)>	14.5
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.999	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6M trisodium citrate, PH6.5