8WDH

Crystal structure of glucose isomerase by fixed-target pink-beam serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	295
Detector technology	PIXEL
Collection date	2023-05-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8366
Spacegroup name	I 2 2 2
Unit cell lengths	95.138, 101.936, 105.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 1.700
R-factor	0.2582
Rwork	0.251
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.861
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.940	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	55523	5313
<I/σ(I)>	4.67
Completeness [%]	100.0
Redundancy	161.7
CC(1/2)	0.824	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	Tris, ammonium sulfate, MgCl2