8WDF

Chemoreceptor PilJ from Pseudomonas aeruginosa PA14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 63
Unit cell lengths	123.434, 123.434, 27.271
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.630 - 2.996
R-factor	0.2681
Rwork	0.260
R-free	0.33610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.451
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.630	3.103
High resolution limit [Å]	2.996	2.996
Rmerge	0.096	1.108
Number of reflections	5037	470
<I/σ(I)>	2.69
Completeness [%]	99.3
Redundancy	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	300	22% PEG 3350, 0.2M Sodium malonate, 4% glycerol