8WDF
Chemoreceptor PilJ from Pseudomonas aeruginosa PA14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 63 |
Unit cell lengths | 123.434, 123.434, 27.271 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.630 - 2.996 |
R-factor | 0.2681 |
Rwork | 0.260 |
R-free | 0.33610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.451 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER (2.7.0) |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.630 | 3.103 |
High resolution limit [Å] | 2.996 | 2.996 |
Rmerge | 0.096 | 1.108 |
Number of reflections | 5037 | 470 |
<I/σ(I)> | 2.69 | |
Completeness [%] | 99.3 | |
Redundancy | 19 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 300 | 22% PEG 3350, 0.2M Sodium malonate, 4% glycerol |