8WD3
The Crystal Structure of JMJD2A(M1-L359) from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.18057 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 51.641, 97.111, 141.083 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.603 - 3.300 |
Rwork | 0.244 |
R-free | 0.29280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.025 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.603 | 3.560 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.162 | 0.675 |
Number of reflections | 11222 | 2264 |
<I/σ(I)> | 6.9 | |
Completeness [%] | 99.8 | |
Redundancy | 5.6 | |
CC(1/2) | 0.996 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M KSCN, 0.1M Bis-Tris propane pH7.5, 20% PEG 3350 |