8WD2
The Crystal Structure of p53 from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.195, 70.889, 105.406 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.712 - 1.850 |
Rwork | 0.174 |
R-free | 0.20810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.570 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.990 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.100 | 0.651 |
Number of reflections | 42141 | 2548 |
<I/σ(I)> | 10.7 | |
Completeness [%] | 99.5 | |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.04M Potassium phosphate monobasic, 16% w/v PEG 8000, 20% v/v Glycerol |