8WB8
Crystal Structure of the shaft pilin LrpA from Ligilactobacillus ruminis - orthorhombic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972423 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.416, 165.240, 47.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.460 - 2.300 |
| R-factor | 0.21789 |
| Rwork | 0.216 |
| R-free | 0.26161 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.253 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.460 | 2.468 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.139 | 0.899 |
| Rmeas | 0.148 | 0.964 |
| Rpim | 0.052 | 0.345 |
| Number of reflections | 15406 | 770 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 94.4 | 91.9 |
| Redundancy | 8.1 | 7.5 |
| CC(1/2) | 0.997 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100 mM Sodium Acetate, pH 4.5, 20% w/v Polyethylene Glycol 3000 |
