8WB8

Crystal Structure of the shaft pilin LrpA from Ligilactobacillus ruminis - orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972423
Spacegroup name	P 21 21 2
Unit cell lengths	62.416, 165.240, 47.928
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.460 - 2.300
R-factor	0.21789
Rwork	0.216
R-free	0.26161
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.253
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.460	2.468
High resolution limit [Å]	2.300	2.300
Rmerge	0.139	0.899
Rmeas	0.148	0.964
Rpim	0.052	0.345
Number of reflections	15406	770
<I/σ(I)>	8.1
Completeness [%]	94.4	91.9
Redundancy	8.1	7.5
CC(1/2)	0.997	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	100 mM Sodium Acetate, pH 4.5, 20% w/v Polyethylene Glycol 3000