8WB3

Human H Chain Ferritin mutant-K86Q with 2 Fe(III)/subunit loading

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-01
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.6314
Spacegroup name	F 4 3 2
Unit cell lengths	181.919, 181.919, 181.919
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.850 - 2.490
R-factor	0.1866
Rwork	0.184
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.864
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.850	2.579
High resolution limit [Å]	2.490	2.490
Number of reflections	17148	921
<I/σ(I)>	14.4
Completeness [%]	99.9
Redundancy	16.2
CC(1/2)	0.998	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM HEPES, pH7, 20 mM CaCl2