8WAV
De novo transcribing complex 12 (TC12), the early elongation complex with Pol II positioned 12nt downstream of TSS
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.72 |
| CTF correction | NONE |
