8WAI
Structure of RvY_06210 at 1.45 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.960, 82.161, 145.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.580 - 1.450 |
R-factor | 0.169 |
Rwork | 0.168 |
R-free | 0.19220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.842 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.960 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.059 | 1.320 |
Rmeas | 0.064 | 1.431 |
Number of reflections | 129382 | 6276 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 99.8 | |
Redundancy | 6.7 | |
CC(1/2) | 0.999 | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.4M Sodium thiocyanate, 0.1M Sodium acetate pH4.0, 16% w/v PEG 4000 |