8WAG

Crystal structure of the C-terminal fragment (residues 716-982) of Arabidopsis thaliana CHUP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-07
Detector	RAYONIX MX300HE
Wavelength(s)	0.90000
Spacegroup name	I 21 3
Unit cell lengths	180.702, 180.702, 180.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.314 - 3.003
R-factor	0.1761
Rwork	0.174
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6L2X
RMSD bond length	0.010
RMSD bond angle	1.288
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.114
Rpim	0.030	0.501
Number of reflections	19727	973
<I/σ(I)>	30.6	2.2
Completeness [%]	99.9
Redundancy	21.7
CC(1/2)		0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM HEPES-NaOH (pH 7.4), 1.1 M sodium malonate, 0.5% (v/v) Jeffamine ED-2001 (pH 7.0)