8WAF
Crystal structure of the C-terminal fragment (residues 756-982 with the C864S mutation) of Arabidopsis thaliana CHUP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97892 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 123.340, 123.340, 160.784 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.486 - 2.800 |
R-factor | 0.2033 |
Rwork | 0.202 |
R-free | 0.22280 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.090 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.201 | |
Rmeas | 0.204 | |
Rpim | 0.032 | 0.512 |
Total number of observations | 770816 | |
Number of reflections | 18407 | 882 |
<I/σ(I)> | 5.5 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 41.9 | |
CC(1/2) | 0.836 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50mM CHES (pH 9.0), 0.2M lithium sulfate, 36% PEG400 |