8W9J
Crystal structure of human CLEC12A ectodomain complexed with 50C1 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2023-05-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 165.356, 45.142, 202.749 |
Unit cell angles | 90.00, 93.13, 90.00 |
Refinement procedure
Resolution | 42.130 - 3.500 |
R-factor | 0.2557 |
Rwork | 0.254 |
R-free | 0.29130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.550 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.540 | 3.830 |
High resolution limit [Å] | 3.500 | 3.500 |
Rpim | 0.115 | 0.328 |
Number of reflections | 19367 | 4549 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 99.3 | |
Redundancy | 3.4 | |
CC(1/2) | 0.981 | 0.773 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES-Na (pH 7.5), 0.2 M sodium chloride, 12% (w/v) PEG8000 |