8W9H

Crystal structure of anti-human CLEC12A antibody 50C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2023-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 41
Unit cell lengths	110.440, 110.440, 76.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.810 - 2.000
R-factor	0.1911
Rwork	0.189
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.629
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.050
High resolution limit [Å]	2.000	2.000
Rpim	0.060	0.501
Number of reflections	62029	4536
<I/σ(I)>	10.8
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.997	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Bis-tris (pH 5.5), 0.2 M ammonium sulfate, 25% (w/v) PEG3350