8W95

Azumapecten Farreri homopolymeric ferritin (ApF) mutant-H2KE exposed to H2O2 for 20 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	I 4 3 2
Unit cell lengths	148.641, 148.641, 148.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.030
R-factor	0.1901
Rwork	0.186
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.104
High resolution limit [Å]	2.030	2.031
Number of reflections	18316	1776
<I/σ(I)>	32.91
Completeness [%]	99.3
Redundancy	37.35
CC(1/2)	0.998	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, pH7, 500 mM NaCl