8W93

Azumapecten Farreri homopolymeric ferritin (ApF) mutant-H2KE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	I 4 3 2
Unit cell lengths	152.679, 152.679, 152.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.180 - 2.090
R-factor	0.1796
Rwork	0.178
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.773
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.180	2.165
High resolution limit [Å]	2.090	2.091
Number of reflections	18289	1776
<I/σ(I)>	18.12
Completeness [%]	99.9
Redundancy	35.8
CC(1/2)	0.999	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, pH7, 500 mM NaCl