8W7Y

Crystal structure of GcCGT in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 32 2 1
Unit cell lengths	63.743, 63.743, 231.812
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.200 - 2.400
R-factor	0.1931
Rwork	0.191
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.606
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.950	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.143	1.435
Rmeas	0.150	1.508
Rpim	0.047	0.458
Total number of observations	212061	24224
Number of reflections	21244	2276
<I/σ(I)>	13.3	1.9
Completeness [%]	94.8
Redundancy	10	10.6
CC(1/2)	0.998	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.2 M Sodium malonate, pH 7.6