8W6Y

Ferritin from Ureaplasma diversum soaking in Fe2+ solution for 10 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.55
Spacegroup name	F 4 3 2
Unit cell lengths	180.513, 180.513, 180.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.910 - 2.502
R-factor	0.2019
Rwork	0.197
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.903
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.257	0.076	2.062
Rmeas	0.259	0.077	2.081
Rpim	0.031	0.010	0.283
Number of reflections	16425	551	441
<I/σ(I)>	2.2
Completeness [%]	100.0	100	100
Redundancy	68.7	57.2	53.3
CC(1/2)	0.997	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	Bicine, MgCl2