8W6W

Crystal Structure of C-terminal domain of nucleocapsid protein from SARS-CoV-2 in complex with ampicillin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-05
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	44.582, 43.604, 56.762
Unit cell angles	90.00, 108.66, 90.00

Refinement procedure

Resolution	24.170 - 2.200
R-factor	0.18225
Rwork	0.180
R-free	0.23827
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yun
RMSD bond length	0.007
RMSD bond angle	1.562
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.170	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	10644	910
<I/σ(I)>	29.4
Completeness [%]	99.8
Redundancy	5.5
CC(1/2)	0.993	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG4000, 0.2 M Lithium sulfate, 50 mM Tris pH 8.0, Ampicillin