8W6F
Apo structure of HBC binder
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-02-08 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.107, 92.402, 51.813 |
| Unit cell angles | 90.00, 108.06, 90.00 |
Refinement procedure
| Resolution | 25.420 - 2.350 |
| R-factor | 0.249 |
| Rwork | 0.247 |
| R-free | 0.27620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.420 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.084 | 0.343 |
| Number of reflections | 14804 | 714 |
| <I/σ(I)> | 20.2 | 4.31 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.1 | 6.1 |
| CC(1/2) | 0.994 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291.15 | 0.2M Lithium sulfate, 20% W/V PEG1000, 0.1M Sodium phosphate citrate PH4.2 |
