8W6F
Apo structure of HBC binder
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2023-02-08 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.107, 92.402, 51.813 |
Unit cell angles | 90.00, 108.06, 90.00 |
Refinement procedure
Resolution | 25.420 - 2.350 |
R-factor | 0.249 |
Rwork | 0.247 |
R-free | 0.27620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.090 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.420 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.084 | 0.343 |
Number of reflections | 14804 | 714 |
<I/σ(I)> | 20.2 | 4.31 |
Completeness [%] | 99.0 | 100 |
Redundancy | 6.1 | 6.1 |
CC(1/2) | 0.994 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291.15 | 0.2M Lithium sulfate, 20% W/V PEG1000, 0.1M Sodium phosphate citrate PH4.2 |