8W6A
Crystal structure of TAX1BP1 LIR region in complex with GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-05-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 |
| Unit cell lengths | 141.160, 141.160, 86.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.680 - 1.530 |
| R-factor | 0.1746 |
| Rwork | 0.174 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.580 | 1.570 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 95523 | 95523 |
| <I/σ(I)> | 27.8 | |
| Completeness [%] | 99.1 | |
| Redundancy | 8.1 | |
| CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 2.8 M Sodium acetate trihydrate and 0.1 M BIS-TRIS propane at pH 7.0 |
