8W5B
Crystal Structure of the shaft pilin LrpA from Ligilactobacillus ruminis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 31 |
| Unit cell lengths | 47.391, 47.391, 227.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.800 - 2.270 |
| R-factor | 0.22332 |
| Rwork | 0.222 |
| R-free | 0.24019 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.264 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.804 | 2.307 |
| High resolution limit [Å] | 2.268 | 2.268 |
| Rmerge | 0.036 | 0.819 |
| Rmeas | 0.038 | 0.860 |
| Rpim | 0.012 | 0.264 |
| Number of reflections | 26024 | 1280 |
| <I/σ(I)> | 27.9 | 2.5 |
| Completeness [%] | 98.4 | 99.7 |
| Redundancy | 10.3 | 10.5 |
| CC(1/2) | 1.000 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M sodium acetate pH 4.6, 15% PEG 3000, 5% polyvinylpyrrolidone K15 |
