8W52

p17 protein structure of HIV2 when OLA1 existing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 2 2 21
Unit cell lengths	123.780, 123.780, 108.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.190 - 2.380
R-factor	0.2245
Rwork	0.210
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.190	2.466
High resolution limit [Å]	2.380	2.381
Rmerge	0.081
Number of reflections	67420	85812
<I/σ(I)>	19.85
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M (NH4)2SO4 0.1M BISTris pH=5.5 25% PEG3350