8W4I
Crystal structure of EndoSz mutant D234M in space group P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-24 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.412, 101.716, 129.243 |
Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
Resolution | 28.300 - 2.900 |
R-factor | 0.2323 |
Rwork | 0.229 |
R-free | 0.27250 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.300 | 3.010 |
High resolution limit [Å] | 2.900 | 2.910 |
Number of reflections | 29373 | 2912 |
<I/σ(I)> | 8.4 | |
Completeness [%] | 99.5 | |
Redundancy | 2 | |
CC(1/2) | 0.991 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium citrate pH 5.4 and 2.2 M ammonium sulfate |