8W44
X-ray crystal structure of V30M-TTR in complex with oxyresveratrol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.226, 84.334, 62.916 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.628 - 1.399 |
R-factor | 0.1532 |
Rwork | 0.152 |
R-free | 0.18120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.890 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.630 | 1.450 |
High resolution limit [Å] | 1.399 | 1.400 |
Rmeas | 0.076 | 0.784 |
Rpim | 0.028 | 0.283 |
Number of reflections | 46133 | 4486 |
<I/σ(I)> | 15.6 | 3.1 |
Completeness [%] | 99.8 | |
Redundancy | 7.3 | |
CC(1/2) | 0.997 | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Disodium malonate, 0.1M NaOAc pH 4.6 |