8W3P

Crystal structure of prefusion-stabilized RSV F protein UFCR3-D(1TD0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	170.713, 170.713, 170.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.250 - 3.200
R-factor	0.2009
Rwork	0.199
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.721
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.250	3.315
High resolution limit [Å]	3.200	3.200
Rmerge	0.084
Number of reflections	11826	463
<I/σ(I)>	31.81
Completeness [%]	80.9
Redundancy	33
CC(1/2)	0.998	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Tri-sodium citrate, 20% PEG 3350