8W3O

Crystal structure of prefusion-stabilized RSV F protein UFCR3-GDQ

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	169.529, 169.529, 169.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.120 - 2.310
R-factor	0.202
Rwork	0.201
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.045
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.120	2.393
High resolution limit [Å]	2.310	2.310
Rmerge	0.137
Number of reflections	36949	3420
<I/σ(I)>	31.5
Completeness [%]	99.3
Redundancy	1
CC(1/2)	0.999	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M potassium thiocyanate, 20% PEG 3350