8W3N

Crystal structure of prefusion-stabilized RSV F protein UFCR3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	168.488, 168.488, 168.488
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.030 - 2.690
R-factor	0.2103
Rwork	0.208
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.142
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.030	2.787
High resolution limit [Å]	2.690	2.690
Number of reflections	23015	2095
<I/σ(I)>	22.32	1.94
Completeness [%]	99.4
Redundancy	5.1
CC(1/2)	0.996	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M di-Sodium tartrate, 18% PEG 3350