8W3L

Crystal structure of prefusion-stabilized RSV F protein UFCR2-iSS-P2-NQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	168.482, 168.482, 168.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.640 - 2.300
R-factor	0.2286
Rwork	0.227
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.418
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.640	2.383
High resolution limit [Å]	2.300	2.300
Number of reflections	36757	3545
<I/σ(I)>	29.43
Completeness [%]	99.8
Redundancy	69.4
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M tri-Potassium citrate, 20% PEG 3350