8W3K

Crystal structure of prefusion-stabilized RSV F protein UFCR2-iSS-NQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	168.749, 168.749, 168.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 2.300
R-factor	0.2521
Rwork	0.251
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.497
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.384
High resolution limit [Å]	2.300	2.300
Number of reflections	36826	3620
<I/σ(I)>	9.27
Completeness [%]	99.8
Redundancy	11.5
CC(1/2)	0.997	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M tri-Lithium citrate, 20% PEG 3350