8W3J

Crystal structure of prefusion-stabilized RSV F protein UFCR2-iSS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	169.220, 169.220, 169.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.840 - 2.830
R-factor	0.226
Rwork	0.224
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.461
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.840	2.930
High resolution limit [Å]	2.830	2.830
Number of reflections	20350	1986
<I/σ(I)>	22.9
Completeness [%]	99.8
Redundancy	30.6
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium Sodium tartrate, 20% PEG 3350