8W3H

Crystal structure of prefusion-stabilized RSV F protein UFCR1-iSS

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	167.933, 167.933, 167.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.550 - 2.250
R-factor	0.2096
Rwork	0.208
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.553
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.550	2.335
High resolution limit [Å]	2.250	2.254
Rmerge	0.141
Number of reflections	38718	3809
<I/σ(I)>	22.67
Completeness [%]	99.9
Redundancy	32.8
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium Sodium tartrate, 16% PEG 3350