8W3G

Crystal structure of prefusion-stabilized RSV F protein UFCR1(1TD0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	168.824, 168.824, 168.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.690 - 2.280
R-factor	0.2247
Rwork	0.223
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.483
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.690	2.362
High resolution limit [Å]	2.280	2.280
Number of reflections	37868	3611
<I/σ(I)>	12.6
Completeness [%]	99.4
Redundancy	21.7
CC(1/2)	0.999	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M tri-Ammonium citrate, 12% PEG 3350