8W3F

Crystal structure of prefusion-stabilized RSV F protein UFCR1-P2-NQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 41 3 2
Unit cell lengths	168.183, 168.183, 168.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.550 - 2.700
R-factor	0.2333
Rwork	0.231
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.533
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	2.797
High resolution limit [Å]	2.700	2.700
Number of reflections	22950	2248
<I/σ(I)>	9.87
Completeness [%]	99.9
Redundancy	16.7
CC(1/2)	0.996	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M tri-Potassium citrate, 20% PEG 3350