8W3E

Crystal structure of prefusion-stabilized RSV F protein UFCR1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 41 3 2
Unit cell lengths	170.033, 170.033, 170.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.250 - 2.260
R-factor	0.2283
Rwork	0.227
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.547
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.250	2.340
High resolution limit [Å]	2.260	2.260
Rmerge	0.181	0.181
Rmeas	0.182
Rpim	0.022	0.021
Number of reflections	39817	39803
<I/σ(I)>	27.5
Completeness [%]	99.9
Redundancy	70.8
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M di-Sodium tartrate, 18% PEG 3350