8W2V
Solution Structure of the CD28 hinge used in chimeric antigen receptor (CAR) T-cells
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE III | 700 | |
| 2 | Bruker | AVANCE III | 800 | |
| 3 | Bruker | AVANCE III | 900 | |
| 4 | Bruker | AVANCE III | 850 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-15N HSQC |
| 10 | 1 | 1 | 3D HNCAN |
| 11 | 1 | 2 | 3D 1H-13C HCCH TOCSY |
| 12 | 1 | 3 | 2D CACO |
| 13 | 1 | 3 | 3D CANCO |
| 14 | 1 | 4 | 3D 1H-15N NOESY |
| 15 | 1 | 5 | 2D 1H-15N HSQC |
| 16 | 1 | 5 | 3D HNHB |
| 17 | 1 | 4 | 2D 1H-15N HSQC |
| 18 | 1 | 2 | 2D 1H-13C HSQC |
| 19 | 1 | 1 | 2D 1H-15N HSQC |
| 2 | 1 | 1 | 2D CON |
| 20 | 1 | 1 | 2D 1H-15N HSQC |
| 21 | 1 | 3 | 2D 1H-15N HSQC |
| 23 | 2 | 4 | 2D 1H-15N HSQC |
| 24 | 2 | 4 | 3D 1H-15N NOESY |
| 25 | 2 | 1 | 2D 1H-15N HSQC |
| 26 | 2 | 1 | 3D HNCO |
| 27 | 2 | 1 | 3D HN(CA)CO |
| 28 | 2 | 2 | 2D 1H-13C HSQC |
| 29 | 2 | 2 | 3D 1H-13C NOESY |
| 3 | 1 | 1 | 3D HNCACB |
| 30 | 2 | 1 | 3D HNCO-COSY |
| 4 | 1 | 1 | 3D CBCA(CO)NH |
| 5 | 1 | 1 | 3D HNCO |
| 6 | 1 | 1 | 3D HN(CA)CO |
| 7 | 1 | 2 | 3D 1H-13C CCH TOCSY |
| 8 | 1 | 2 | 3D 1H-13C NOESY |
| 9 | 1 | 2 | 2D 1H-13C HSQC |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | 1 | 298 |
| 2 | 6.5 | 1 | 284 |
Conformers
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 25 |
