8W2P
BsaXI-DNA complex I
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA,FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200,3000 |
| Cs (mm) | 2.7 |
| Phase Plate | VOLTA PHASE PLATE,OTHER |
| Detector | DIRECT ELECTRON DE-10 (5k x 4k),DIRECT ELECTRON DE-20 (5k x 3k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.14 |
| Software | cryoSPARC,PHENIX,SerialEM,Coot |
| Symmetry | C1 |
| CTF correction | NONE |
