8W1F

Crystal Structure of DPS-like protein PA4880 from Pseudomonas aeruginosa (dodecamer, Mg bound)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	215.790, 215.790, 277.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.270 - 3.000
R-factor	0.1804
Rwork	0.179
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5156: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.940	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.202	1.798
Rmeas	0.209	1.861
Rpim	0.055	0.476
Total number of observations	1874799	96579
Number of reflections	130805	6414
<I/σ(I)>	12.1	1.8
Completeness [%]	100.0
Redundancy	14.3	15.1
CC(1/2)	0.997	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	Salt Rx G2: 1 M magnesium sulfate, 100 mM Bis-Tris pH 7.0