8W1E

Crystal Structure of DPS-like protein PA4880 from Pseudomonas aeruginosa (dodecamer)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	216.178, 216.178, 278.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.860 - 2.900
R-factor	0.1786
Rwork	0.177
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.191	1.790
Rmeas	0.198	1.852
Rpim	0.052	0.475
Total number of observations	2082925	107710
Number of reflections	145618	7128
<I/σ(I)>	12.8	1.7
Completeness [%]	100.0
Redundancy	14.3	15.1
CC(1/2)	0.998	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Salt Rx F9: 800 mM lithium sulfate, 100 mM Tris pH 8.5