8W0W
Crystal structure of broadly neutralizing antibody hcab64 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2021-07-21 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.680, 88.231, 174.709 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.990 - 2.130 |
R-factor | 0.1963 |
Rwork | 0.195 |
R-free | 0.22410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.082 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.350 | 2.190 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.163 | 3.063 |
Rmeas | 0.179 | 3.342 |
Rpim | 0.071 | 1.320 |
Total number of observations | 365813 | 28522 |
Number of reflections | 59648 | 4582 |
<I/σ(I)> | 7.2 | 1.1 |
Completeness [%] | 99.1 | |
Redundancy | 6.1 | 6.2 |
CC(1/2) | 0.995 | 0.366 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 8% Tacsimate and 20% PEG 3,350 |