8W05
Crystal Structure of the reconstruction of the ancestral triosephosphate isomerase of the last opisthokont common ancestor obtained by maximum likelihood
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-06 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.416, 83.597, 126.016 |
| Unit cell angles | 90.00, 105.77, 90.00 |
Refinement procedure
| Resolution | 43.800 - 2.160 |
| R-factor | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.938 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 2.200 |
| High resolution limit [Å] | 2.160 | 2.165 |
| Number of reflections | 113623 | 5644 |
| <I/σ(I)> | 15.6 | 2.05 |
| Completeness [%] | 98.2 | |
| Redundancy | 4.4 | 2.7 |
| CC(1/2) | 0.905 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.2 M Sodium malonate pH 5.0, 20% (w/v) PEG 3350 |
