8VZY
Q108K:K40L:T51V:T53S:R58Y mutant of hCRBPII bound to synthetic fluorophore TD-1V
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 |
Unit cell lengths | 32.289, 35.252, 64.872 |
Unit cell angles | 92.51, 90.49, 111.72 |
Refinement procedure
Resolution | 29.200 - 1.340 |
Rwork | 0.238 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qyn |
RMSD bond length | 0.013 |
RMSD bond angle | 1.632 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.200 | 1.370 |
High resolution limit [Å] | 1.340 | 1.340 |
Number of reflections | 47165 | 12003 |
<I/σ(I)> | 43.37 | |
Completeness [%] | 79.6 | |
Redundancy | 2 | |
CC(1/2) | 0.837 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG4000, ammonium acetate, sodium acetate |