8VZX
Q108K:K40L:T53A:R58F mutant of hCRBPII bound to synthetic fluorophore TD-1V
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-17 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 |
Unit cell lengths | 36.451, 55.167, 69.876 |
Unit cell angles | 109.94, 98.49, 101.41 |
Refinement procedure
Resolution | 27.400 - 1.470 |
Rwork | 0.233 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qyn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.283 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.400 | 1.510 |
High resolution limit [Å] | 1.470 | 1.470 |
Number of reflections | 82805 | 16538 |
<I/σ(I)> | 32.9 | |
Completeness [%] | 95.7 | |
Redundancy | 2.9 | |
CC(1/2) | 0.896 | 0.912 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | ammonium acetate, sodium acetate, PEG4000 |