8VXN

Human Bcl-2/Bcl-xL Chimera Fused to Maltose-Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-10
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.000000
Spacegroup name	P 21 21 21
Unit cell lengths	37.070, 88.950, 140.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.240 - 2.090
R-factor	0.2064
Rwork	0.204
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.553
Data reduction software	autoPROC (1.1.7)
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.222	2.040
High resolution limit [Å]	2.005	2.005
Rmerge	0.079	0.821
Rmeas	0.086	0.890
Rpim	0.034	0.338
Number of reflections	29956	1591
<I/σ(I)>	13	2.1
Completeness [%]	93.0	99.8
Redundancy	6.3	6.8
CC(1/2)	0.998	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100 mM Tris (pH 8.0), 29% PEG 3350, 200 mM sodium chloride