8VXK

Crystal Structure of the Apo Bacillus subtilis GabR C-terminal Effector-binding and Oligomerization Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12718
Spacegroup name	P 41
Unit cell lengths	118.782, 118.782, 75.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 1.850
R-factor	0.2078
Rwork	0.196
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.177
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.970	1.880
High resolution limit [Å]	1.850	1.850
Number of reflections	84313	33025
<I/σ(I)>	9.2	1.5
Completeness [%]	94.8	100
Redundancy	7.1	7.4
CC(1/2)	0.992	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	6% PEG 6000, 100mM HEPES pH 7.0