8VXB

Structure of ANT(6)-Ib from Campylobacter fetus subsp fetus complexed with hydrated streptomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-09
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979493
Spacegroup name	P 31 2 1
Unit cell lengths	73.305, 73.305, 127.572
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.000 - 1.850
R-factor	0.1731
Rwork	0.172
R-free	0.19770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.074
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	127.570	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.339	4.272
Rmeas	0.347	4.376
Rpim	0.076	0.945
Total number of observations	708684	104983
Number of reflections	34637	4977
<I/σ(I)>	10.6	1.9
Completeness [%]	100.0
Redundancy	20.5	21.1
CC(1/2)	0.998	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	0.1 M sodium malate, 10 % PEG 3350, 10 mM HEPES