8VWL

Crystal structure of Vibrio cholerae NFeoB in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	77
Detector technology	PIXEL
Collection date	2021-11-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	48.348, 84.118, 158.028
Unit cell angles	76.17, 83.91, 74.51

Refinement procedure

Resolution	77.570 - 3.670
R-factor	0.2115
Rwork	0.209
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.619
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.590	3.730
High resolution limit [Å]	3.670	3.670
Number of reflections	24215	1142
<I/σ(I)>	7
Completeness [%]	95.0
Redundancy	1.9
CC(1/2)	0.998	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG 3350, 0.2 M magnesium chloride, 0.1 M bis-tris pH 5.5