8VVB
Influenza antibody L5A7 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.760, 109.190, 140.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.020 - 1.780 |
| R-factor | 0.1746 |
| Rwork | 0.174 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.843 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.089 | 1.919 |
| Rmeas | 0.093 | 2.451 |
| Rpim | 0.026 | 1.485 |
| Number of reflections | 141507 | 3715 |
| <I/σ(I)> | 18.7 | 0.5 |
| Completeness [%] | 94.2 | 50.5 |
| Redundancy | 11.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 17.5% PEG 4000, 0.2M ammonium acetate, 0.1M sodium citrate, pH 5.6 |
