8VVA

Structure of DUF1480 domain-containing protein from Klebsiella pneumoniae subsp. pneumoniae SA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	78.591, 78.591, 62.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.530 - 1.750
R-factor	0.2082
Rwork	0.205
R-free	0.23910
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	20271	20271
<I/σ(I)>	14.1
Completeness [%]	99.7
Redundancy	11.1
CC(1/2)	0.971	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	25% PEG 3350/0.1M citric acid pH 3.5